Biophotonics

Raman spectroscopy shows great promise to supplement and possibly replace standard histopathological diagnosis. We have demonstrated the ability to discriminate between healthy primary human keratinocytes (black line), primary human keratinocytes infected with the E7 vector of HPV-16, which represents an extremely early stage of neoplastic development (blue line) and finally CasKi cells, an advanced cervical cancer derived cell line (red line). We are currently characterising the Raman spectra of other cancer cell types. One dream is that during surgery, suspect tissue might be immediately diagnosed in situ.

Key References

Modulated Raman Spectroscopy for enhanced identification of bladder tumour cells in urine samples, Elisabetta Canetta, Michael Mazilu, Anna Chiara De Luca, Antonia E. Carruthers, Kishan Dholakia, Sam Neilson, Harry Sargeant, Tina Briscoe, C. Simon Herrington, and Andrew C. Riches, accepted for publication in the Journal of Biomedical Optics (2011)

Enhanced bioanalyte detection in waveguide confined Raman spectroscopy using modulation techniques, P.C. Ashok, A.C.D. Luca, M. Mazilu, and K. Dholakia, (article online in advance of print) Journal of Biophotonics (2011)

Waveguide confined Raman spectroscopy for microfluidic interrogation, Praveen C. Ashok, Gajendra P. Singh, Helen A. Rendall, Thomas F. Krauss  and Kishan Dholakia (article online in advance of print) Lab on a Chip (2011).

Optimal algorithm for fluorescence suppression of modulated Raman spectroscopy, Michael Mazilu, Anna Chiara De Luca, Andrew Riches, C. Simon Herrington, and Kishan Dholakia, Optics Express 18, 11382-11395 (2010)

Near-Infrared Raman Spectroscopy Using Hollow-Core Photonic Bandgap Fibers, K. M. Tan, G. P. Singh, C. S. Herrington, and C. T. A. Brown, Optics Commun. 283(16) 3204-3206 (2010)

Discrimination of normal from pre-malignant cervical tissue by Raman mapping of de-paraffinized histological tissue sections, Khay M. Tan, C. Simon Herrington, Christian T. A. Brown, Journal of Biophotonics (online in advance of print) (2010)

Fiber probe based microfluidic raman spectroscopy, P. C. Ashok, G. P. Singh, K. M. Tan, and K. Dholakia, Optics Express 18(8) 7642-7649 (2010) (Highlighted in optics.org)

On-line fluorescence suppression in modulated Raman spectroscopy, Anna Chiara De Luca, Michael Mazilu, Andrew Riches, Simon C. Herrington, Kishan Dholakia. Analytical Chemistry 82(2) 738-745 (2010) (BBC news article) (Daily Express news article).

(If desired, all references and pdfs may be downloaded en masse in an Endnote formatted zip file)